Summary
Type: TLC
Application #: 400510
Catalog#: 1624026 - Silica G TLC Plates, polyester backed, 200um, 20x20cm 25/pk
Detection: temperate 5 min at 90 °C, then spray with iodoplatinate reagent (see visualization reagents)
Conditions: Eluent: 2-dimensional development:
1st dimension: ethyl acetate / methanol / water (7:2:1, v/v/v)
2nd dimension: chloroform / acetone / ammonia (5:94:1, v/v/v)
Urine samples with 10 μg/ml each of ecgonine and benzoylecgonine and control urine samples are pretreated as described, applied at the origin X and developed in two dimensions. The figure shows A) unspecified urine constituents, B) butylated ecgonine , C) butylated benzoylecgonine, D) morphine . hRf values in the cited eluent system: morphine = 23, butylated ecgonine = 83. Detection limit 3 μg/ml urine each.
Authors: Bastos M L
Source: J Chromatogr 89 (1974) 335